two-photon microscope movable objective microscope (mom) Search Results


two-photon benchtop microscope  (Femtonics inc)
90
Femtonics inc two-photon benchtop microscope
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Two Photon Benchtop Microscope, supplied by Femtonics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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photon microscope  (Nikon)
99
Nikon photon microscope
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Photon Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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photon microscope - by Bioz Stars, 2026-09
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two-photon laser scanning microscopy ti-sapphire laser  (TPLSM laboratories)
90
TPLSM laboratories two-photon laser scanning microscopy ti-sapphire laser
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Two Photon Laser Scanning Microscopy Ti Sapphire Laser, supplied by TPLSM laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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two-photon laser scanning microscopy ti-sapphire laser - by Bioz Stars, 2026-09
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two photon laser scanning microscope  (Bio-Rad)
91
Bio-Rad two photon laser scanning microscope
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Two Photon Laser Scanning Microscope, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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two photon laser scanning microscope - by Bioz Stars, 2026-09
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two-photon microscope dermainspect  (JenLab GmbH)
90
JenLab GmbH two-photon microscope dermainspect
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Two Photon Microscope Dermainspect, supplied by JenLab GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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two-photon microscope dermainspect - by Bioz Stars, 2026-09
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2d two-photon laser scanning microscope  (Femtonics inc)
90
Femtonics inc 2d two-photon laser scanning microscope
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
2d Two Photon Laser Scanning Microscope, supplied by Femtonics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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two-photon laser scanning microscope femto3d-rc  (Femtonics inc)
90
Femtonics inc two-photon laser scanning microscope femto3d-rc
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Two Photon Laser Scanning Microscope Femto3d Rc, supplied by Femtonics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-photon+microscope+movable+objective+microscope+(mom)/femto3d+rc+imaging+system/10__1523_slash_eneuro__0416___22__2022-84-26-31
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two-photon laser scanning microscope femto3d-rc - by Bioz Stars, 2026-09
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two-photon laser scanning microscopy  (Femtonics inc)
90
Femtonics inc two-photon laser scanning microscopy
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Two Photon Laser Scanning Microscopy, supplied by Femtonics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-photon+microscope+movable+objective+microscope+(mom)/two+photon+laser+scanning+microscopy/10__1523_slash_jneurosci__0393___24__2024-64-5-9
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femto2d two-photon microscope  (Femtonics inc)
90
Femtonics inc femto2d two-photon microscope
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Femto2d Two Photon Microscope, supplied by Femtonics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-photon+microscope+movable+objective+microscope+(mom)/femto2d+laser+scanning+microscope/pmc11353153-128-8-10
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femto2d two-photon microscope - by Bioz Stars, 2026-09
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two-photon laser scanning microscopy  (TPLSM laboratories)
90
TPLSM laboratories two-photon laser scanning microscopy
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Two Photon Laser Scanning Microscopy, supplied by TPLSM laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-photon+microscope+movable+objective+microscope+(mom)/two+photon+laser+scanning+microscopy/pmc05957003-6-14-18
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two-photon laser scanning microscopy - by Bioz Stars, 2026-09
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two photon microscope  (Scientifica)
86
Scientifica two photon microscope
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Two Photon Microscope, supplied by Scientifica, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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twophoton resonant galvo scanning microscope  (Thorlabs)
86
Thorlabs twophoton resonant galvo scanning microscope
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Twophoton Resonant Galvo Scanning Microscope, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon benchtop microscope. Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.

Journal: bioRxiv

Article Title: All-viral tracing of monosynaptic inputs to single birthdate-defined neurons in the intact brain

doi: 10.1101/2021.10.18.464781

Figure Lengend Snippet: A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon benchtop microscope. Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.

Article Snippet: Mice were imaged with a custom two-photon benchtop microscope (Femtonics, Hungary) or two-photon miniscope ( Zong et al ., 2017, 2021 ; Obenhaus et al ., 2021 ).

Techniques: Injection, In Utero, Expressing, Staining, In Vivo Imaging, Imaging, Microscopy, Activity Assay